{"title":"Cell-Free Protein Expression","description":"\u003cp\u003eCleavion Cell-Free Protein Expression includes lyophilized and standard cell-free protein synthesis kits for in vitro expression without living cells. This category is useful for rapid testing, construct screening and protein production workflow development.\u003c\/p\u003e\u003cp\u003eShopify filters should focus on system type, reaction count, lyophilized format and pack size. Keep this collection as a kit\/workflow page rather than mixing these products with standalone enzymes.\u003c\/p\u003e","products":[{"product_id":"cell-free-protein-synthesis-kit-lyophilized","title":"Cell-Free Protein Synthesis Kit (Lyophilized)","description":"The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription\/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg\/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: This product is not suitable for the soluble expression of disulfide-rich proteins, as it does not provide the oxidative environment required for correct disulfide bond folding. If needed, please contact us for other REF NO..","brand":"Cleavion","offers":[{"title":"20 Reactions","offer_id":57188444537207,"sku":"CLV-0233","price":105.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0233-0-cleavion-product.png?v=1781696423"},{"product_id":"cell-free-protein-synthesis-kit-lyophilized-1","title":"Cell-Free Protein Synthesis Kit (Lyophilized)","description":"The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription\/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg\/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: This product is not suitable for the soluble expression of disulfide-rich proteins, as it does not provide the oxidative environment required for correct disulfide bond folding. If needed, please contact us for other REF NO..","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188444766583,"sku":"CLV-0234","price":309.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0234-0-cleavion-product.png?v=1781696423"},{"product_id":"cell-free-protein-synthesis-kit-pro-lyophilized","title":"Cell-Free Protein Synthesis Kit Pro (Lyophilized)","description":"The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription\/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg\/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: This product contains the chaperone proteins GroEL (~60 kDa) and GroES (~10 kDa), representing the most extensively characterized chaperone system currently under research. They create a closed folding chamber that provides an optimal environment for unfolded proteins, reducing misfolding and aggregation, thereby increasing the probability of soluble expression. However, protein solubility is influenced by multiple factors (e.g., inherent protein structure, expression system, reaction conditions, etc.) . While this product can significantly improve folding efficiency, soluble expression is not guaranteed for all proteins","brand":"Cleavion","offers":[{"title":"20 Reactions","offer_id":57188444897655,"sku":"CLV-0235","price":140.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0235-0-cleavion-product.png?v=1781696424"},{"product_id":"cell-free-protein-synthesis-kit-pro-lyophilized-1","title":"Cell-Free Protein Synthesis Kit Pro (Lyophilized)","description":"The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription\/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg\/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: This product contains the chaperone proteins GroEL (~60 kDa) and GroES (~10 kDa), representing the most extensively characterized chaperone system currently under research. They create a closed folding chamber that provides an optimal environment for unfolded proteins, reducing misfolding and aggregation, thereby increasing the probability of soluble expression. However, protein solubility is influenced by multiple factors (e.g., inherent protein structure, expression system, reaction conditions, etc.) . While this product can significantly improve folding efficiency, soluble expression is not guaranteed for all proteins","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445061495,"sku":"CLV-0236","price":438.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0236-0-cleavion-product.png?v=1781696425"},{"product_id":"cell-free-protein-synthesis-kit-max-lyophilized","title":"Cell-Free Protein Synthesis Kit Max (Lyophilized)","description":"The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription\/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg\/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: The product is suitable for expressing diverse proteins, particularly disulfide-rich proteins.","brand":"Cleavion","offers":[{"title":"20 Reactions","offer_id":57188445258103,"sku":"CLV-0237","price":170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0237-0-cleavion-product.png?v=1781696425"},{"product_id":"cell-free-protein-synthesis-kit-max-lyophilized-1","title":"Cell-Free Protein Synthesis Kit Max (Lyophilized)","description":"The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription\/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg\/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: The product is suitable for expressing diverse proteins, particularly disulfide-rich proteins.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445454711,"sku":"CLV-0238","price":568.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0238-0-cleavion-product.png?v=1781696426"}],"url":"https:\/\/quantimol.myshopify.com\/collections\/cell-free-protein-expression.oembed","provider":"Cleavion","version":"1.0","type":"link"}