{"title":"Cloning \u0026 DNA Assembly","description":"\u003cp\u003eCleavion Cloning \u0026amp; DNA Assembly groups ready-to-use reagents for seamless DNA assembly, multi-fragment cloning and assembly-based molecular biology workflows. These products are best presented as workflow reagents rather than standalone enzymes.\u003c\/p\u003e\u003cp\u003eThis collection should help customers identify assembly format, reaction count and compatible cloning strategy. Products in this category can also carry enzyme-family tags when relevant, but the collection copy should stay focused on the complete cloning workflow.\u003c\/p\u003e","products":[{"product_id":"dna-assembly-mix-mono","title":"DNA Assembly Mix Mono","description":"DNA Assembly Mix Mono is a kind of seamless assembly method, digest, ligate or end repair are not required. This method has been used to assemble different sizes of DNA fragments with varied overlaps (15~30 bp) . And it offers a easy, fast and efficient DNA cloning technique. DNA Assembly Mix Mono is specifically designed for single-fragment DNA assembly. It can complete single-fragment recombination in as fast as 5 minutes with a positive rate of over 95%. Compared with DNA Assembly Mix Ultra (REF: EG24204), DNA Assembly Mix Mono does not rely on ligase, has simpler components, and is more compatible with unpurified PCR products. Meanwhile, DNA Assembly Mix Mono contains no ligase and relies on the repair system of E. coli, thus ensuring higher fidelity.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188441620855,"sku":"CLV-0195","price":74.61,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0195-0-cleavion-product.png?v=1781696387"},{"product_id":"dna-assembly-mix-ultra","title":"DNA Assembly Mix Ultra","description":"DNA Assembly Mix Ultra is a kind of seamless assembly methoddigest, ligate or end repair are not required. This method has beenused to assemble different sizes of DNA fragments with variedoverlaps (15~30 bp) . And it offers a easy, fast and efficient DNA cloningtechnique. The DNA Assembly Mix Ultra allows for the assembly of one ormultiple DNA fragments in a single reaction, with single-fragmentinsertion completed in as little as 5 minutes and a positive rateexceeding 95%.This Mix uses HiFi Taq DNA Ligase, which hashigher fidelity compared to wild-type Taq DNA Ligase, significantlyimproving the success rate of seamless cloning. Additionally, the Mixincludes a transformation enhancer, greatly increasing the number oftransformants. While ensuring high fidelity and transformation efficiencythe DNA Assembly Mix Ultra has optimized its components to enhancestability, making it more stable under high temperature or oxidicconditions.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188441686391,"sku":"CLV-0176","price":74.61,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0176-0-cleavion-product.png?v=1781696387"},{"product_id":"dna-assembly-mix-mono-1","title":"DNA Assembly Mix Mono","description":"DNA Assembly Mix Mono is a kind of seamless assembly method, digest, ligate or end repair are not required. This method has been used to assemble different sizes of DNA fragments with varied overlaps (15~30 bp) . And it offers a easy, fast and efficient DNA cloning technique. DNA Assembly Mix Mono is specifically designed for single-fragment DNA assembly. It can complete single-fragment recombination in as fast as 5 minutes with a positive rate of over 95%. Compared with DNA Assembly Mix Ultra (REF: EG24204), DNA Assembly Mix Mono does not rely on ligase, has simpler components, and is more compatible with unpurified PCR products. Meanwhile, DNA Assembly Mix Mono contains no ligase and relies on the repair system of E. coli, thus ensuring higher fidelity.","brand":"Cleavion","offers":[{"title":"250 Reactions","offer_id":57188441817463,"sku":"CLV-0196","price":166.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0196-0-cleavion-product.png?v=1781696388"},{"product_id":"golden-gate-dna-assembly-kit-with-bpii","title":"Golden Gate DNA Assembly Kit with BpiI","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BpiI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188442571127,"sku":"CLV-0281","price":482.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0281-0-cleavion-product.png?v=1781696413"},{"product_id":"golden-gate-dna-assembly-kit-with-bpii-1","title":"Golden Gate DNA Assembly Kit with BpiI","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BpiI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"10 Reactions","offer_id":57188445389175,"sku":"CLV-0280","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0280-0-cleavion-product.png?v=1781696425"}],"url":"https:\/\/quantimol.myshopify.com\/collections\/cloning-dna-assembly.oembed","provider":"Cleavion","version":"1.0","type":"link"}