{"title":"Reverse Transcription \u0026 cDNA Synthesis","description":"\u003cp\u003eCleavion Reverse Transcription \u0026amp; cDNA Synthesis products support conversion of RNA templates into cDNA for downstream PCR, qPCR and cloning workflows. The range includes standalone reverse transcriptases and ready-to-use first-strand synthesis mixes.\u003c\/p\u003e\u003cp\u003eThis collection should help users choose between enzyme-only products and complete reaction mixes. Recommended filters include enzyme type, reaction count, DNase inclusion, pack size and application.\u003c\/p\u003e","products":[{"product_id":"m-mlv-rnase-h-reverse-transcriptase","title":"M-MLV RNase H-Reverse Transcriptase","description":"M-MLV RNase H- Reverse Transcriptase is a genetically modified and recombinant RNase H-deficient Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase. Through genetic engineering modification, the optimal reaction temperature of this enzyme has been increased to 42 °C , and its extension ability is stronger, making it suitable for longer cDNA synthesis.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188435820919,"sku":"CLV-0033","price":69.71,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0033-0-cleavion-product.png?v=1781696342"},{"product_id":"m-mlv-giii-reverse-transcriptase","title":"M-MLV GIII Reverse Transcriptase","description":"M-MLV GIII Reverse Transcriptase is third-generation reverse transcriptase derived through genetic engineering and recombinant technology from the Moloney Murine Leukemia Virus (M-MLV) . This enzyme has been modified to eliminate the RNase H activity. Additionally, it exhibits significantly improved thermal stability (optimal reaction temperature:50 °C) . These greatly improve the specificity and length of the synthesized first-strand cDNA, allowing for the synthesis of cDNA fragments up to 12 kb. Moreover, M-MLV GIII Reverse Transcriptase shows enhanced tolerance to RNA secondary structures.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188435951991,"sku":"CLV-0034","price":86.96,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0034-0-cleavion-product.png?v=1781696342"},{"product_id":"all-in-one-first-strand-synthesis-master-mix-with-dsdnase","title":"All-in-One First-Strand Synthesis Master Mix (with dsDNase)","description":"The All-in-One First-Strand Synthesis MasterMix (with dsDNase) is a comprehensive kit for first-strand cDNA synthesis, containing reverse transcriptase, reaction buffer, RNAse inhibitor, dNTPs, Oligo (dT) 20VN, and random primers. It provides all the necessary components for efficient cDNA synthesis and requires only the addition of RNA template and water to initiate the reaction. Within just 15 minutes, cDNA fragments up to 12 kb in length can be obtained, which can be utilized downstream in experiments such as qPCR and conventional PCR. This kit incorporates dsDNase, which effectively removes genomic DNA contamination. Unlike conventional DNase I, dsDNase specifically digests double-stranded DNA (dsDNA) and hybrid DNA-RNA molecules while exhibiting thermal sensitivity, enabling rapid and irreversible inactivation under high-temperature conditions. Compared to traditional methods that employ DNase I to remove genomic DNA contamination, the use of dsDNase eliminates the need for additional EDTA for inactivation. This not only saves experimental time but also reduces inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436115831,"sku":"CLV-0035","price":83.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0035-0-cleavion-product.png?v=1781696343"},{"product_id":"all-in-one-first-strand-synthesis-master-mix","title":"All-in-One First-Strand Synthesis Master Mix","description":"All-in-One First-Strand Synthesis MasterMix is a high-quality, efficient, and convenient one-step cDNA synthesis mix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including thermostable M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, Oligo (dT) 20VN, and random primers-all the necessary components. Simply add RNA template and water to initiate the reaction. Using the reverse transcription pre-mix, cDNA of up to 12 kb in size can be obtained within 15 minutes.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436246903,"sku":"CLV-0036","price":86.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0036-0-cleavion-product.png?v=1781696344"},{"product_id":"high-accuracy-reverse-transcriptase","title":"High-Accuracy Reverse Transcriptase","description":"The High-Accuracy Reverse Transcriptase is a modified version of M-MLV GIII Reverse Transcriptase that utilizes nucleic acid aptamer technology. It is covalently linked to the M-MLV enzyme and exhibits suppressed activity below 31°C, thereby enhancing the specificity of the enzyme's products. The optimal reaction temperature for this enzyme is 50°C, and it possesses enhanced extension capabilities, making it suitable for synthesizing longer cDNA fragments.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188436345207,"sku":"CLV-0037","price":157.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0037-0-cleavion-product.png?v=1781696344"},{"product_id":"rtase-iii-primer-flexible-all-in-one-mix-with-dsdnase","title":"RTase III Primer Flexible All-in-One Mix (with dsDNase)","description":"RTase III Primer Flexible All-in-One Mix is a high-quality, efficient, and convenient one-step cDNA synthesis premix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, and random primers-all the necessary components. Additionally, it requires the addition of RNA templates, primers, and water to initiate the reaction. This kit can be used with different types of reverse transcription primers to meet diverse experimental needs. Depending on the experimental design, Oligo (dT) 20VN, Random hexamers, or Gene Specific Primers can be selected. This reverse transcription premix allows for the generation of cDNA up to 12 kb in size within 15 minutes. RNA extracted from cells often contains genomic DNA contamination. If the genomic DNA is not removed before reverse transcription, both the genomic DNA and cDNA will be amplified during downstream qPCR reactions (especially when the primers are designed on the same exon), thus affecting the accuracy of gene expression quantification. This kit utilizes dsDNase to efficiently remove genomic DNA contamination. dsDNase can specifically digest double-stranded DNA (dsDNA or the DNA strand in DNA-RNA hybrid chains) and is thermally sensitive, rapidly and irreversibly inactivated at the reverse transcription temperature. Compared to the traditional method of using DNase I to remove genomic DNA contamination, dsDNase does not require the addition of EDTA for inactivation, saving experimental time and reducing inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436476279,"sku":"CLV-0038","price":93.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0038-0-cleavion-product.png?v=1781696346"},{"product_id":"m-mlv-giv-reverse-transcriptase","title":"M-MLV GIV Reverse Transcriptase","description":"M-MLV GIV Reverse Transcriptase is a fourth-generation M-MLV reverse transcriptase obtained through genetic modification and recombination technologies. Compared with the wild-type M-MLV reverse transcriptase, it lacks RNase H activity. Key enhancements include superior thermal stability, with an optimal reaction temperature of 55°C and sustained 50% activity at 70°C. Furthermore, it demonstrates improved tolerance to complex secondary structures , accelerated reaction speed (completing reactions in as little as 10 minutes), and high template affinity. These combined properties ensure robust performance with low-copy templates and enable efficient synthesis of full-length cDNA fragments up to 20 kb.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188436607351,"sku":"CLV-0039","price":93.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0039-0-cleavion-product.png?v=1781696346"}],"url":"https:\/\/quantimol.myshopify.com\/collections\/reverse-transcription-cdna-synthesis.oembed","provider":"Cleavion","version":"1.0","type":"link"}