{"product_id":"2x-taq-plus-pcr-master-mix","title":"2x Taq-Plus PCR Master Mix","description":"\u003cstyle\u003e\n  .clv-seo-block {\n    --clv-navy: #06184a;\n    --clv-blue: #0b2f78;\n    --clv-cyan: #00c8f5;\n    --clv-cyan-soft: #e8fbff;\n    --clv-ice: #f6fcff;\n    --clv-text: #223047;\n    --clv-muted: #667085;\n    --clv-line: #d7edf5;\n    font-family: Arial, Helvetica, sans-serif;\n    color: var(--clv-text);\n    line-height: 1.65;\n    background: #ffffff;\n  }\n\n  .clv-seo-block * {\n    box-sizing: border-box;\n  }\n\n  .clv-seo-wrap {\n    max-width: 1180px;\n    margin: 0 auto;\n    padding: 28px 18px 46px;\n  }\n\n  .clv-hero {\n    background: linear-gradient(135deg, var(--clv-navy), var(--clv-blue) 55%, #063d76);\n    padding: 34px;\n    color: #ffffff;\n    position: relative;\n    overflow: hidden;\n    box-shadow: 0 18px 45px rgba(6, 24, 74, 0.18);\n    border-left: 6px solid var(--clv-cyan);\n  }\n\n  .clv-hero:after {\n    content: \"\";\n    position: 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.clv-cta {\n      padding: 22px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"clv-seo-block\"\u003e\n\u003cdiv class=\"clv-seo-wrap\"\u003e\n\u003csection class=\"clv-hero\"\u003e\u003cspan class=\"clv-eyebrow\"\u003ePCR Polymerase Master Mix\u003c\/span\u003e\n\u003ch2\u003e2x Taq-Plus PCR Master Mix for Routine PCR Amplification\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is a ready-to-use PCR premix designed for convenient, consistent DNA amplification. The 2x formulation combines Taq DNA Polymerase, reaction buffer, dNTPs, and optimized PCR components in one tube, reducing preparation time and helping improve repeatability across routine molecular biology workflows.\u003c\/p\u003e\n\u003cdiv class=\"clv-badges\"\u003e\n\u003cspan class=\"clv-badge\"\u003eSKU: CLV-0305\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFormat: 5 × 1 mL\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eStorage: -20 °C\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFor PCR products up to 5 kb\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"clv-grid\"\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eReady-to-use 2x PCR premix\u003c\/h3\u003e\n\u003cp\u003eThe master mix format simplifies reaction setup by reducing the number of individual reagents that must be pipetted into each PCR tube.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eOptimized for routine PCR\u003c\/h3\u003e\n\u003cp\u003eSuitable for standard endpoint PCR, colony screening, template verification, insert checking, and general DNA amplification tasks.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eDirect T\/A cloning compatibility\u003c\/h3\u003e\n\u003cp\u003ePCR products generated with Taq-based amplification can be used for downstream T\/A cloning workflows when compatible with the selected cloning system.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003c\/div\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e2x Taq-Plus PCR Master Mix is formulated for researchers who need reliable PCR amplification with fewer preparation steps. Because the mix already contains polymerase, buffer, dNTPs, and supporting reaction components, users only need to add template DNA, primers, and nuclease-free water to prepare the final PCR reaction.\u003c\/p\u003e\n\u003cp\u003eThis PCR master mix is especially useful for laboratories running repeated PCR assays, screening multiple samples, or standardizing reaction setup between users. The premixed format helps reduce pipetting variation and supports efficient setup of single reactions or larger sample batches.\u003c\/p\u003e\n\u003cdiv class=\"clv-two-col\"\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eRecommended Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine PCR amplification\u003c\/li\u003e\n\u003cli\u003eDNA fragment amplification up to 5 kb\u003c\/li\u003e\n\u003cli\u003eColony PCR and clone screening\u003c\/li\u003e\n\u003cli\u003eGenotyping PCR workflows\u003c\/li\u003e\n\u003cli\u003eTemplate confirmation and insert verification\u003c\/li\u003e\n\u003cli\u003ePreparation of PCR products for T\/A cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eKey Advantages\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient 2x master mix format\u003c\/li\u003e\n\u003cli\u003eReduced pipetting steps during PCR setup\u003c\/li\u003e\n\u003cli\u003eConsistent reagent composition between reactions\u003c\/li\u003e\n\u003cli\u003eGood amplification specificity for routine targets\u003c\/li\u003e\n\u003cli\u003eCompatible with common molecular biology workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable class=\"clv-spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003e2x Taq-Plus PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog \/ SKU\u003c\/th\u003e\n\u003ctd\u003eCLV-0305\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003ePCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCategory\u003c\/th\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSubcategory\u003c\/th\u003e\n\u003ctd\u003eConventional DNA polymerase \u0026amp; PCR mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzyme System\u003c\/th\u003e\n\u003ctd\u003eTaq DNA Polymerase-based PCR premix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e2x ready-to-use liquid master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e5 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmplification Range\u003c\/th\u003e\n\u003ctd\u003eDesigned for PCR products up to 5 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Conditions\u003c\/th\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-highlight\"\u003e\n\u003ch2\u003eHow to Use 2x Taq-Plus PCR Master Mix\u003c\/h2\u003e\n\u003cp\u003eThe 2x concentration is intended to be diluted to 1x in the final PCR reaction. In a typical workflow, combine the master mix with forward primer, reverse primer, DNA template, and nuclease-free water according to the final reaction volume required by your assay.\u003c\/p\u003e\n\u003cdiv class=\"clv-workflow\"\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e1. Thaw \u0026amp; mix\u003c\/strong\u003e Bring the master mix to working temperature and mix gently before use.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e2. Add primers\u003c\/strong\u003e Add forward and reverse primers according to your validated PCR design.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e3. Add template\u003c\/strong\u003e Add DNA template and nuclease-free water to reach the final reaction volume.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e4. Run PCR\u003c\/strong\u003e Use cycling conditions suitable for the template, primer pair, and expected amplicon length.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eWhy Choose Cleavion 2x Taq-Plus PCR Master Mix?\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is designed for laboratories that need a practical PCR reagent for everyday amplification tasks. The premixed format supports efficient preparation, helps reduce manual reagent handling, and allows researchers to focus on template quality, primer design, and PCR optimization.\u003c\/p\u003e\n\u003cp\u003eFor workflows such as clone screening, routine gene amplification, sample verification, and T\/A cloning preparation, this Taq-based PCR master mix offers a convenient balance of usability and performance.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-faq\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdiv class=\"clv-faq-grid\"\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is 2x Taq-Plus PCR Master Mix used for?\u003c\/h3\u003e\n\u003cp\u003eIt is used for routine PCR amplification of DNA fragments. Common applications include endpoint PCR, colony PCR, clone screening, genotyping workflows, and insert verification.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat does “2x” mean in a PCR master mix?\u003c\/h3\u003e\n\u003cp\u003e“2x” means the master mix is supplied at twice the final working concentration. It is normally combined with primers, template DNA, and water so that the final PCR reaction contains the master mix at 1x concentration.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat components are included in the master mix?\u003c\/h3\u003e\n\u003cp\u003eThe mix contains Taq DNA Polymerase, PCR reaction buffer, dNTPs, and optimized components required for standard PCR setup. Users add primers, DNA template, and nuclease-free water.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eCan PCR products from this mix be used for T\/A cloning?\u003c\/h3\u003e\n\u003cp\u003eYes. PCR products generated with this Taq-based master mix can be directly used for T\/A cloning workflows when the downstream cloning system is compatible.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is the recommended storage temperature?\u003c\/h3\u003e\n\u003cp\u003eStore Cleavion 2x Taq-Plus PCR Master Mix at -20 °C. Avoid unnecessary freeze-thaw cycles and mix gently before use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat amplicon size can this master mix amplify?\u003c\/h3\u003e\n\u003cp\u003eThe product is designed to amplify DNA fragments up to 5 kb, depending on template quality, primer design, GC content, and PCR cycling conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-cta\"\u003e\n\u003ch2\u003eCleavion PCR Reagents for Molecular Biology Workflows\u003c\/h2\u003e\n\u003cp\u003eUse 2x Taq-Plus PCR Master Mix when you need a convenient, Taq-based PCR premix for routine amplification, screening, and cloning-related workflows. Its ready-to-use 2x format helps simplify setup while supporting consistent PCR performance across repeated assays.\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"2x Taq-Plus PCR Master Mix\",\n  \"sku\": \"CLV-0305\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Cleavion\"\n  },\n  \"category\": \"PCR Master Mix\",\n  \"description\": \"Cleavion 2x Taq-Plus PCR Master Mix is a ready-to-use Taq DNA Polymerase-based PCR premix containing reaction buffer, dNTPs, and optimized components for routine PCR amplification up to 5 kb.\",\n  \"url\": \"https:\/\/cleavion.com\/products\/2x-taq-plus-pcr-master-mix\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Catalog number\",\n      \"value\": \"CLV-0305\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Format\",\n      \"value\": \"2x ready-to-use PCR master mix\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack size\",\n      \"value\": \"5 × 1 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Storage conditions\",\n      \"value\": \"-20 °C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Application\",\n      \"value\": \"Routine PCR amplification, colony PCR, clone screening, genotyping, T\/A cloning preparation\"\n    }\n  ]\n}\n\u003c\/script\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188446011767,"sku":"CLV-0305","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0305-0-cleavion-product.png?v=1781696440","url":"https:\/\/quantimol.myshopify.com\/products\/2x-taq-plus-pcr-master-mix","provider":"Cleavion","version":"1.0","type":"link"}