{"product_id":"aapcas12b-crispr-nuclease","title":"AapCas12b CRISPR Nuclease","description":"AapCas12b is a Type V Cas nuclease, exhibiting good activity across 37~60°C. Guided by crRNA and tracrRNA (or sgRNA), it exhibits cis-cleavage activity against both double-stranded and single-stranded DNA targets. For double-stranded DNA targets, AapCas12b recognizes the site complementary to the crRNA (or sgRNA) spacer sequence downstream of the PAM sequence (5'-TTN) in target DNA, specifically cleaves the target double-stranded DNA to generate sticky ends. For single-stranded DNA targets, its specific cleavage is PAM-independent. After AapCas12b, target DNA and sgRNA form a ternary complex, the enzyme exhibits trans-cleavage activity, namely non-specific cleavage of single-stranded DNA with any sequence. AapCas12b exhibits good activity in common isothermal amplification buffers, making it suitable for rapid nucleic acid detection.","brand":"Cleavion","offers":[{"title":"100 pmol","offer_id":57188442734967,"sku":"CLV-0282","price":137.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0282-0-cleavion-product.png?v=1781696415","url":"https:\/\/quantimol.myshopify.com\/products\/aapcas12b-crispr-nuclease","provider":"Cleavion","version":"1.0","type":"link"}