{"product_id":"bbvci-restriction-enzyme","title":"BbvCI Restriction Enzyme","description":"\u003cp\u003e\u003cstrong\u003eBbvCI\u003c\/strong\u003e is derived from the \u003cem\u003eBbvCI\u003c\/em\u003e gene of \u003cem\u003eBacillus brevis\u003c\/em\u003e and is obtained through recombinant expression in \u003cem\u003eEscherichia coli\u003c\/em\u003e. BbvCI is a Type IIA restriction enzyme that exists as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site.\u003c\/p\u003e\n\u003cp\u003eIt recognizes and cleaves the non-palindromic sequence \u003cstrong\u003eCCTCAGC (-5\/-2)\u003c\/strong\u003e, generating \u003cstrong\u003e5′ cohesive ends with 3-base overhangs\u003c\/strong\u003e. BbvCI uses the \u003cstrong\u003erecommended reaction buffer\u003c\/strong\u003e and is compatible with double digestion with other \u003cstrong\u003erapid-digestion restriction enzymes\u003c\/strong\u003e. However, extended digestion exceeding \u003cstrong\u003e3 hours\u003c\/strong\u003e should be avoided to reduce the risk of star activity.\u003c\/p\u003e","brand":"Cleavion","offers":[{"title":"50 U","offer_id":57188442046839,"sku":"CLV-0139","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0139-0-cleavion-product.png?v=1781696389","url":"https:\/\/quantimol.myshopify.com\/products\/bbvci-restriction-enzyme","provider":"Cleavion","version":"1.0","type":"link"}