{"product_id":"exonuclease-iii","title":"Exonuclease III","description":"Exonuclease III is a nuclease that stepwise removes mononucleotides from the 3'-OH termini of double-stranded DNA (dsDNA), and inactive on single-stranded DNA (ssDNA) . The preferred substrates of Exonuclease III are blunt or recessed 3'-termini, although the enzyme also acts at nick sites in double-stranded DNA to generate single-stranded gaps. 3'-overhanging termini are resistant to cleavage; the degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This characteristic can be used to produce strand-specific ssDNA with designed linearized dsDNA having one non-cleavage end (3'-overhang) and the other end designed as a cleavable end (blunt or 5'-overhang), allowing Exonuclease III to digest only one strand. Exonuclease III also exhibits RNase H, 3'-phosphatase, and apurinic\/apyrimidinic-endonuclease activities.","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57094766920055,"sku":"CLV-0166","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0166-0-cleavion.png?v=1778840202","url":"https:\/\/quantimol.myshopify.com\/products\/exonuclease-iii","provider":"Cleavion","version":"1.0","type":"link"}