Ready-to-use 2x PCR premix
The master mix format simplifies reaction setup by reducing the number of individual reagents that must be pipetted into each PCR tube.
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2x Taq-Plus PCR Master Mix
2x Taq-Plus PCR Master Mix
SKU:CLV-0305
Regular price
$98.30 USD
Regular price
$42.00 USD
Sale price
$98.30 USD
Taxes included.
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Product Specifications
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Description
Taq-Plus PCR Master Mix (2x) is a PCR reaction premix formulated with Taq DNA Polymerase. It already contains reaction buffer, dNTPs, and other components, providing convenience and efficiency. This mix could amplify up to 5 kb, exhibiting excellent amplification specificity and template compatibility. The PCR products can be directly used for T/A cloning.
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Category
Polymerase
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Sub Category
Conventional DNA polymerase & PCR mix
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Components
-20 °C
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Storage Conditions
-20 °C
2x Taq-Plus PCR Master Mix
2x Taq-Plus PCR Master Mix for Routine PCR Amplification
Cleavion 2x Taq-Plus PCR Master Mix is a ready-to-use PCR premix designed for convenient, consistent DNA amplification. The 2x formulation combines Taq DNA Polymerase, reaction buffer, dNTPs, and optimized PCR components in one tube, reducing preparation time and helping improve repeatability across routine molecular biology workflows.
SKU: CLV-0305 Format: 5 × 1 mL Storage: -20 °C For PCR products up to 5 kb
Optimized for routine PCR
Suitable for standard endpoint PCR, colony screening, template verification, insert checking, and general DNA amplification tasks.
Direct T/A cloning compatibility
PCR products generated with Taq-based amplification can be used for downstream T/A cloning workflows when compatible with the selected cloning system.
Product Overview
2x Taq-Plus PCR Master Mix is formulated for researchers who need reliable PCR amplification with fewer preparation steps. Because the mix already contains polymerase, buffer, dNTPs, and supporting reaction components, users only need to add template DNA, primers, and nuclease-free water to prepare the final PCR reaction.
This PCR master mix is especially useful for laboratories running repeated PCR assays, screening multiple samples, or standardizing reaction setup between users. The premixed format helps reduce pipetting variation and supports efficient setup of single reactions or larger sample batches.
Recommended Applications
- Routine PCR amplification
- DNA fragment amplification up to 5 kb
- Colony PCR and clone screening
- Genotyping PCR workflows
- Template confirmation and insert verification
- Preparation of PCR products for T/A cloning
Key Advantages
- Convenient 2x master mix format
- Reduced pipetting steps during PCR setup
- Consistent reagent composition between reactions
- Good amplification specificity for routine targets
- Compatible with common molecular biology workflows
Technical Specifications
| Product Name | 2x Taq-Plus PCR Master Mix |
|---|---|
| Catalog / SKU | CLV-0305 |
| Product Type | PCR master mix |
| Category | Polymerase |
| Subcategory | Conventional DNA polymerase & PCR mix |
| Enzyme System | Taq DNA Polymerase-based PCR premix |
| Format | 2x ready-to-use liquid master mix |
| Pack Size | 5 × 1 mL |
| Amplification Range | Designed for PCR products up to 5 kb |
| Storage Conditions | -20 °C |
How to Use 2x Taq-Plus PCR Master Mix
The 2x concentration is intended to be diluted to 1x in the final PCR reaction. In a typical workflow, combine the master mix with forward primer, reverse primer, DNA template, and nuclease-free water according to the final reaction volume required by your assay.
1. Thaw & mix Bring the master mix to working temperature and mix gently before use.
2. Add primers Add forward and reverse primers according to your validated PCR design.
3. Add template Add DNA template and nuclease-free water to reach the final reaction volume.
4. Run PCR Use cycling conditions suitable for the template, primer pair, and expected amplicon length.
Why Choose Cleavion 2x Taq-Plus PCR Master Mix?
Cleavion 2x Taq-Plus PCR Master Mix is designed for laboratories that need a practical PCR reagent for everyday amplification tasks. The premixed format supports efficient preparation, helps reduce manual reagent handling, and allows researchers to focus on template quality, primer design, and PCR optimization.
For workflows such as clone screening, routine gene amplification, sample verification, and T/A cloning preparation, this Taq-based PCR master mix offers a convenient balance of usability and performance.
Frequently Asked Questions
What is 2x Taq-Plus PCR Master Mix used for?
It is used for routine PCR amplification of DNA fragments. Common applications include endpoint PCR, colony PCR, clone screening, genotyping workflows, and insert verification.
What does “2x” mean in a PCR master mix?
“2x” means the master mix is supplied at twice the final working concentration. It is normally combined with primers, template DNA, and water so that the final PCR reaction contains the master mix at 1x concentration.
What components are included in the master mix?
The mix contains Taq DNA Polymerase, PCR reaction buffer, dNTPs, and optimized components required for standard PCR setup. Users add primers, DNA template, and nuclease-free water.
Can PCR products from this mix be used for T/A cloning?
Yes. PCR products generated with this Taq-based master mix can be directly used for T/A cloning workflows when the downstream cloning system is compatible.
What is the recommended storage temperature?
Store Cleavion 2x Taq-Plus PCR Master Mix at -20 °C. Avoid unnecessary freeze-thaw cycles and mix gently before use.
What amplicon size can this master mix amplify?
The product is designed to amplify DNA fragments up to 5 kb, depending on template quality, primer design, GC content, and PCR cycling conditions.
Cleavion PCR Reagents for Molecular Biology Workflows
Use 2x Taq-Plus PCR Master Mix when you need a convenient, Taq-based PCR premix for routine amplification, screening, and cloning-related workflows. Its ready-to-use 2x format helps simplify setup while supporting consistent PCR performance across repeated assays.