AvrII Restriction Enzyme

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Restriction Endonucleases

LightNing® Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne ® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit.

5 U/μL

CCTAGG 5'...C ↓ C T A G G...3' 3'...G G A T C ↑ C...5'

AspA2I, BlnI, XmaJI

• LightNing® AvrII (5 U/μl) : 25 μl (75 U) • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

-20°C

2~8°C