PmlI Restriction Enzyme

Rapid-digestion restriction enzymes are engineered restriction enzymes designed for fast DNA digestion. These enzymes show strong activity in a universal digestion buffer and can digest DNA within 5–15 minutes. This enables different combinations of restriction enzymes to work simultaneously in a single reaction tube and reduces the need for sequential digestion steps.

Rapid-digestion restriction enzymes undergo strict quality control testing and can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. The color loading digestion buffer contains a density reagent together with red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion. In a 1% agarose gel, the red dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.

Restriction Endonucleases

Rapid-Digestion Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoKI methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.

20 U/μL

CACGTG 5'...CAC ↓ GTG...3' 3'...GTG ↑ CAC ..5'

AcvI, Eco72I, PspCI, BbrPI

• Rapid-digestion PmlI (20 U/μl): 100 μl / 2,000 U
• 10× Universal Digestion Buffer: 1 ml
• 10× Color Loading Digestion Buffer: 1 ml

-20°C

2~8°C