• Poly(A) RNA Polymerase for mRNA Tailing
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Poly(A) RNA Polymerase for mRNA Tailing

Poly(A) RNA Polymerase for mRNA Tailing

CLV-0288

$140.00 USD
$72.00 USD $140.00 USD
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  • Description

    Poly (A) Polymerase is a polymerase derived from Escherichia coli, also known as E.coli PAP, PAP enzyme, Poly (A) tailing enzyme, or PolyA tailing enzyme. Poly (A) Polymerase catalyzes the rapid and efficient addition of a poly (A) tail to the 3' end of single-stranded RNA (ssRNA) in the presence of ATP. It can use various single-stranded RNA as substrate, while double-stranded RNA and excessively short oligonucleotides (<20 nt) are not recommended as substrates. DNA cannot be used as substrates for this enzyme. Poly (A) tailing reaction catalyzed by E. coli Poly (A) Polymerase can only use ATP, but not ADP or dATP. In addition, when CTP and UTP are used, the incorporation amount is less than 5% of that when using ATP. GTP can not be used by this enzyme.

  • Category

    In Vitro Transcription

  • Sub Category

    In Vitro Transcription

  • Storage Conditions

    Store at -20°C for 2 years.

Poly(A) RNA Polymerase for mRNA Tailing

Poly (A) Polymerase is a polymerase derived from Escherichia coli, also known as E.coli PAP, PAP enzyme, Poly (A) tailing enzyme, or PolyA tailing enzyme. Poly (A) Polymerase catalyzes the rapid and efficient addition of a poly (A) tail to the 3' end of single-stranded RNA (ssRNA) in the presence of ATP. It can use various single-stranded RNA as substrate, while double-stranded RNA and excessively short oligonucleotides (<20 nt) are not recommended as substrates. DNA cannot be used as substrates for this enzyme. Poly (A) tailing reaction catalyzed by E. coli Poly (A) Polymerase can only use ATP, but not ADP or dATP. In addition, when CTP and UTP are used, the incorporation amount is less than 5% of that when using ATP. GTP can not be used by this enzyme.