PspGI (EcoRII) Restriction Enzyme

Rapid-digestion restriction enzymes are engineered restriction enzymes designed for fast DNA digestion. These enzymes show strong activity in a universal digestion buffer and can digest DNA within 5–15 minutes. This enables different combinations of restriction enzymes to work simultaneously in a single reaction tube and reduces the need for sequential digestion steps.

Rapid-digestion restriction enzymes undergo strict quality control testing and can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. The color loading digestion buffer contains a density reagent together with red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion. In a 1% agarose gel, the red dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.

Restriction Endonucleases

Rapid-Digestion Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 75°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dcm methylase.

10 U/μL

CCWGG 5'... ↓ C C W G G ...3' 3'... G G W C C ↑ ...5'

EcoRII, Psp6I, AjnI

• Rapid-digestion PspGI (10 U/μl): 200 μl / 2,000 U
• 10× Universal Digestion Buffer: 2 × 1 ml
• 10× Color Loading Digestion Buffer: 2 × 1 ml

-20°C

2~8°C