SfiI Restriction Enzyme

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Restriction Endonucleases

LightNing® Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 50°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dcm methylase.

20 U/μL

GGCCNNNNNGGCC 5'...G G C C N N N N ↓ N G G C C...3' 3'...C C G G N ↑ N N N N C C G G...5'

• LightNing® SfiI (20 U/μl) : 100 μl (2000 U) • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

-20°C

2~8°C